DNA Polymerases
As One International Inc Taq DNA Polymerase 5 U/ul glycerol free, 10x Ammonium Buffer and 10x Standard Buffer both Magnesium free, 10,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Glycerol free Taq is ideal for automation and free drying applications. Included with Taq are magnesium free 10x Ammonium Buffer and 10x Standard Buffer.
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer, 2,500U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes 10x Ammonium Buffer and 10x Combination Buffer
As One International Inc Hot Start Taq Polymerase 5U/ul, 10x Ammonium Buffer and 10x Combination Buffer both Magnesium free, 1,000U
AS ONE HS DNA Polymerase is a modified form of AS ONE Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Polymerase DNA Polymerase into the reaction. HS Taq includes magnesium free 10x Ammonium Buffer and 10x Combination Buffer
As One International Inc Taq DNA Polymerase 1.1x Master Mix, 1.5 mM MgCl2 (final concentration), 5,000Rxn
AS ONE's Taq DNA Polymerase Pre-Mix is a ready-to-use 1.1X reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. AS ONE Taq DNA Polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are present in Taq DNA Polymerase Mix. Each reaction requires 45 uL of the 1.1X reaction mix. Simply add primers, template and water to a total reaction volume of 50 uL.Taq DNA Polymerase Pre-Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
As One International Inc Taq DNA Polymerase 5 U/ul, 10x Ammonium Buffer and 10x Combination buffer both Magnesium and detergent free , 2,500U
AS ONE's Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes Tween/Triton/Magnesium free 10x Ammonium Buffer and 10x Combination Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Standard Buffer, 2,500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq is 10x Standard Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Ammonium Buffer, Tween free, 500U
AS ONE Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. AS ONE Taq DNA Polymerase has both a 5 prime to 3 prime DNA polymerase and a 5 prime to 3 prime' exonuclease activity. The enzyme lacks a 3 prime to 5 prime exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for TA cloning. Included with Taq is magnesium free 10x Ammonium Buffer.
INTACT GENOMICS INC Bsu DNA Polymerase Large Fragment, Glycerol Free 2000 Units (5 units/µl)
Glycerol Free Bsu DNA Polymerase I, Large Fragment is suitable for various purposes including RPA (Recombinase Polymerase Amplification).Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.
INTACT GENOMICS INC Bsu DNA Polymerase Large Fragment, Glycerol Free 1000 Units (5 units/µl)
Glycerol Free or Lyophilized Bsu DNA Polymerase I, Large Fragment is suitable for various purposes including RPA (Recombinase Polymerase Amplification).Bsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity. This large fragment also lacks 3´→ 5´ exonuclease activity.
INTACT GENOMICS INC T4 UvsX DNA Recombinase, Lyophilized 500µg
Lyophilized T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. Glycerol Free T4 UvsX DNA Recombinase is useful for Recombinase Polymerase Amplification (RPA) and other applications.Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/RAD51 family perform the central catalytic role in this process. UvsX recombinase is the RecA/Rad51 ortholog of bacteriophage T4. T4 UvsX DNA Recombinase and other recombinases form presynaptic filaments on ssDNA that are activated to search for homology in dsDNA and to perform DNA strand exchange.
ABclonal Technology Klenow Fragment 3'to5' exo-
Klenow Fragment (3 to 5 exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5 to 3 exonuclease activity and has mutations (D355A, E357A) which abolish the 3 to 5 exonuclease activity. Klenow Fragment (3 to 5 exo-) is isolated from a recombinant source. It generates probes using random primers and shows moderate strand displacement activity. It can be used in random primer labeling, DNA sequencing by the Sanger dideoxy method, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Standard Buffer, Magnesium free, 2,500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes magnesium free 10x Standard Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Standard Buffer, 5,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes 10x Standard Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Standard Buffer, Magnesium free, 500U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes magnesium free 10x Standard Buffer.
As One International Inc Red-Taq DNA Polymerase 5 U/ul 10x Standard Buffer, 1,000U
AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. Taq with Red Dye is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase. AS ONE Taq DNA Polymerase with red dye has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity. Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Red Taq Polymerase includes 10x Standard Buffer.